16-P003 Evc and Evc2 are interacting cilia transmembrane proteins that modulate Ihh signalling
نویسندگان
چکیده
Marc A. Willaredt, Kerstin Hasenpusch-Theil, Humphrey Gardner, Igor Kitanovic, Vera Hirschfeld-Warneken, Christian Gojak, Karin Gorgas, C. Lulu Bradford, Joachim Spatz, Stefan Woelfl, Thomas Theil, Kerry L. Tucker 1 Interdisciplinary Center for Neurosciences, University of Heidelberg, Heidelberg, Germany 2 Dept. of Anatomy, University of Heidelberg, Heidelberg, Germany 3 Centres for Neuroscience Research and Integrative Physiology, University of Edinburgh, Edinburgh, United Kingdom 4 Novartis Institutes forBioMedicalResearch, Cambridge, MA, United States 5 Institute of Pharmacy and Molecular Biotechnology, University of Heidelberg, Heidelberg, Germany 6 Institute for Physical Chemistry, Dept. of Biophysical Chemistry, University of Heidelberg, Heidelberg, Germany 7 Max Planck Institute for Metals Research, Stuttgart, Germany
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We have developed anti-Evc2 antibodies and show that Evc2 and Evc co-localise at the basal body and also in primary cilia. In transfected cells, basal body and cilia localisation are only observed when Evc and Evc2 constructs are co-transfected but do not occur when either construct is transfected individually indicating that this localisation is co-dependent. Although Evc localises to cilia it...
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UNLABELLED The Hedgehog (Hh) pathway depends on primary cilia in vertebrates, but the signaling machinery within cilia remains incompletely defined. We report the identification of a complex between two ciliary proteins, EFCAB7 and IQCE, which positively regulates the Hh pathway. The EFCAB7-IQCE module anchors the EVC-EVC2 complex in a signaling microdomain at the base of cilia. EVC and EVC2 ge...
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Ellis-van Creveld syndrome (EvC; OMIM 225500) is a recessive disorder comprising chondrodysplasia, polydactyly, nail dysplasia, orofacial abnormalities and, in a proportion of patients, cardiovascular malformations. Weyers acrodental dysostosis (Weyers; OMIM 193530) is an allelic dominant disorder comprising polydactyly, nail dysplasia, and orofacial abnormalities. EvC results from loss-of-func...
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ورودعنوان ژورنال:
- Mechanisms of Development
دوره 126 شماره
صفحات -
تاریخ انتشار 2009